Isolation, identification, and monitoring of antibiotic resistance in Pasteurella multocida and Mannheimia haemolytica isolated from sheep in East Azerbaijan province, Iran

Authors

  • A.R. Jabbari Department of Aerobic Bacterial Vaccines, Razi Vaccine and Serum Research Institute, Karaj, Iran
  • GH. Shokri Department of Aerobic Bacterial Vaccines, Razi Vaccine and Serum Research Institute, Karaj, Iran
  • I. Khalili Virus Biobank Laboratory, Razi Vaccine and Serum Research Institute, Karaj, Iran
  • M. Ebrahimi Technical Director of Veterinary Vaccines, Razi Vaccine and Serum Research Institute, Karaj, Iran
  • N. Razmaraii Department of Research and Development, Razi Vaccine and Serum Research Institute, Karaj, Iran
  • R. Ghaderi Department of Aerobic Bacterial Vaccines, Razi Vaccine and Serum Research Institute, Karaj, Iran
  • R. Ghadimipour Departent ofm Parasite Vaccine Research and Production, Razi Vaccine and Serum Research Institute, Karaj, Iran Department of Research and Development, Razi Vaccine and Serum Research Institute, Karaj, Iran
Abstract:

The present study was carried out in order to isolate, identify, and assess the antimicrobial susceptibility of the causative agent(s) of pneumonic pasteurellosis in sheep in East Azerbaijan province, northwest of Iran. Pneumonia was detected in 320 cases, and the affected lungs were sampled in the slaughterhouse. The samples were investigated bacteriologically for the isolation of two microorganisms from the Pasteurellaceae family. Pasteurella multocida was isolated from six (1.87%) samples, while none of the lung tissues were positive for Mannheimia haemolytica. After the isolation and detection of microorganisms via cultural and morphological tests, the bacteria were identified on the basis of biochemical criteria and polymerase chain reaction (PCR) technique. Antimicrobial susceptibility testing was performed on all P. multocida isolates, using broth microdilution method. Evaluation of the minimum inhibitory concentration (MIC) of eight antimicrobial agents against the tested isolates showed that all the organisms were resistant to amoxicillin and relatively susceptible to ceftiofur. In conclusion, P. multocida was introduced as the main cause of ovine pneumonic pasteurellosis in the studied district, and the outbreak frequency significantly varied in different seasons of the year (P

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

isolation, identification, and monitoring of antibiotic resistance in pasteurella multocida and mannheimia haemolytica isolated from sheep in east azerbaijan province, iran

the present study was carried out in order to isolate, identify, and assess the antimicrobial susceptibility of the causative agent(s) of pneumonic pasteurellosis in sheep in east azerbaijan province, northwest of iran. pneumonia was detected in 320 cases, and the affected lungs were sampled in the slaughterhouse. the samples were investigated bacteriologically for the isolation of two microorg...

full text

Isolation and Identification of Pasteurella multocida by PCR from sheep and goats in Fars province, Iran

During one year period from 2010 to 2011 the samples from pneumonic animals were taken and transported to the laboratory. Pasteurella multocida were identified in 16.6% of animal by biochemical test. The high incidence of P. multocida was obtained in the south of Fars province, where the area was warm region. The Mean Death Time between the isolates was 12-18 and 19-24 hours. Only the capsular ...

full text

Isolation and Identification of Pasteurella multocida from Sheep & Goat in Iran

This study has been carried out with the objective of isolation and identification of agent(s) of pasteurella pneumonia in sheep and goat in Iran using bacteriological and biochemical assays to be identified in the pursuant researches to be used in pasteurellosis vaccine production. To accomplish this objective, samples were gathered from areas suspicious to pasteurellosis infection and industr...

full text

Antimicrobial susceptibility testing of Mannheimia haemolytica and Pasteurella multocida isolated from calves with dairy calf pneumonia

  This study evaluated the nasopharyngeal microbial flora and antimicrobial susceptibility patterns of the one hundred and thirty Holstein calves with dairy calf pneumonia from dairy farms of Mashhad Suburb between September 2002 and August 2003 . The most common micro-organisms isolated were Pasteurella multocida 80 (61.54%), Mannheimia haemolytica 41 (31.54%), Bacillus sp. 15 (11.54%), Staphy...

full text

isolation and identification of pasteurella multocida by pcr from sheep and goats in fars province, iran

during one year period from 2010 to 2011 the samples from pneumonic animals were taken and transported to the laboratory. pasteurella multocida were identified in 16.6% of animal by biochemical test. the high incidence of p. multocida was obtained in the south of fars province, where the area was warm region. the mean death time between the isolates was 12-18 and 19-24 hours. only the capsular ...

full text

Molecular identification and capsular typing of Pasteurella multocida isolates from sheep pneumonia in Iran

Pasteurella multocida is known as one of the main organisms causing pneumonia in sheep. As immunity in pasteurellosis is serogroup specific, identification of prevalent capsular group among endemic areas is essential. The aim of this study was to molecular identification and determine of the capsular type of the P. multocida strains isolated from sheep pneumonia in Iran. Bacteriological and bio...

full text

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 71  issue 3

pages  153- 160

publication date 2016-09-01

By following a journal you will be notified via email when a new issue of this journal is published.

Keywords

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023